Composite

Part:BBa_K3763003:Design

Designed by: Liran Mao   Group: iGEM21_WHU-China   (2021-10-18)


pFadD promoter with LacI repressor regulating downstream GFP with RBS BBa_K3763000


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 30
    Illegal suffix found in sequence at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 30
    Illegal SpeI site found at 231
    Illegal PstI site found at 245
    Illegal NotI site found at 36
    Illegal NotI site found at 238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 30
    Illegal suffix found in sequence at 231
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 30
    Illegal XbaI site found at 45
    Illegal SpeI site found at 231
    Illegal PstI site found at 245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 938


Design Notes

FENG, Youjun; CRONAN, John E. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PloS one, 2012, 7.9: e46275.


Source

Designed by our team members on the basis of BBa_K3040014, FENG, Youjun; CRONAN, John E. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PloS one, 2012, 7.9: e46275. and https://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection


References